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Studies consistently show that deficiencies in key minerals like selenium, zinc, and copper significantly compromise antioxidant defenses and increase vulnerability to oxidative damage across multiple body systems

Other studies have suggested that heme modifies cardiac contractile proteins through posttranslational protein modifications by consolidating with myosin light chain 1, which results in serious contractile heart dysfunction ( 2+ sensitivity (pCa50) ( 2+ homeostasis, and impairs systolic function ( In vitro , heme-treated adult rat cardiomyocytes have shown a significant reduction in systolic Ca 2+ transient amplitudes ( 2+ -handling proteins, such as the ryanodine receptor2 (RyR2) and the sarcoendoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) (Khler et al., 2014), and may have activated intracellular stress kinases, such as calcium/calmodulin-dependent protein kinase II (CaMKII) ( 2+ mishandling

doi: 10.3390/ijms222111359 218 SetiadiA

These assays use synthetic probes that react selectively with glutathione to generate a fluorescent or luminescent signal proportional to analyte concentration