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Fluorophores were excited using 405 nm (DAPI), 488 nm (BODIPY 493/503), and 633 nm (Alexa Fluor 647) laser lines, and emission was collected using standard DAPI, FITC/GFP, and Cy5 detection channels

The chemical structure of cyanocobalamin contains the rare mineral cobalt (4.34%), which binds the cyano group and is located in the center of a corrin ring

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