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After a night of adherent growth, DPCs were treated with 10 7 M DHT and 10 5 M finasteride (Solarbio, Beijing, China) for the next 48 h, respectively

Nat Neurosci 9:331339 Riquelme PA, Drapeau E, Doetsch F (2008) Brain micro-ecologies: neural stem cell niches in the adult mammalian brain

To better monitor the fate of internalized cholesterol once it reaches LE/Lys in drug-treated NPC1 mutants and further reduce endogenous cholesterol contributing to background staining, cells were lipid-starved in 10% LPDS for 48 h, prior to cholesterol loading with 10% FCS in the presence or absence of 50 M lead compound 10 for additional 24 h