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In accordance with the instructions for use of the rat organ tissue lymphocyte separation solution, the IELs and the LPLs were separated from the above single cells by density gradient centrifugation

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Astrocytes, which preferentially accumulate Mn due to the presence of high-affinity Mn transporters (Sidoryk-Wegrzynowicz and Aschner, 2013), are primarily responsible for the glutamate uptake via GLAST and GLT (Rothstein et al., 1996