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Hematoxylin and eosin staining was employed for general histopathological evaluation, while Nissl staining was used to assess neuronal integrity

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The evolution of treatment outcomes for resected stage IIIA non-small cell lung cancer over 16 years at a single institution

For H 2 O 2 assessment in isolated mitochondria, 50 g protein-aliquots of freshly obtained mitochondria were incubated with 100 M AmplexRed probe (Invitrogen) in mitochondrial respiration buffer (125 mM KCl, 2 mM KH 2 PO 4 , 1 mM MgCl 2 , 0.5 mg ml 1 bovine serum albumin and 10 mM HEPES, pH 7.4) containing horseradish peroxidase (0.5 U ml 1 )