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RNA-seq raw read counts for each gene model were analyzed using edgeR, an R package for data normalization (CalcNormFactors), using the TMM method to correct for compositional bias in RNA-seq data, filtering (filterByExpr) to remove lowly expressed genes with low statistical power in the analysis, exploration (plotMDS), and differential gene expression (DEG) identification [41]

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Smoking in primary headaches a systematic review and meta-analysis