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The suspended liver microsomes were placed on ice in prechilled microcentrifuge tubes, allowing for the addition of 640 ul of 0.1 M Tris buffer and 10 ul of 500 uM test compound to the sample

Pharmacists may also add in other components, such as vitamin B12, that can change the way the active drug works in the body

Researchers select format based on route of administration requirements and whether the protocol calls for fixed or variable dosing across experimental arms