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Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

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In this blend they are already combined, and the pairing has a mechanistic rationale: NAD+ supplies the coenzyme, while 5-Amino-1MQ blocks the NNMT enzyme that would otherwise drain NAD+ precursors [4]

Insights into eukaryogenesis from the fossil record