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Thrombin was removed by Benzamidine-Sepharose 6B resin (Cytiva) and tag-free TbTR was further purified by reverse immobilized metal affinity chromatography (Protino Ni-TED resin, Macherey-Nagel)

Statements Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

Acetyl-DL-carnitine levels were significantly higher in untreated R cells than WT cells (p-value = 5.8 10 12 ), whereas hexanoyl-L-carnitine was significantly higher in untreated WT cells than R (p-value = 6.6 10 9 )

Triamterene-induced hyperkalemia can cause atrial fibrillation, supraventricular tachycardia (SVT) and life-threatening cardiac arrhythmias such as ventricular fibrillation, AV block, and ventricular tachycardia