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Role of NLRP3 inflammasome/caspase 1 signaling pathway in cyclophosphamide-induced ovarian injury Inflammasomes, which are multiprotein complexes, assemble in the cytoplasm and are triggered by many endogenous and exogenous stimuli, including ROS and DAMPs

Membranes were incubated with primary antibodies overnight at 4 C, followed by HRP-conjugated GAPDH Rabbit mAb (#AC054, 1:5000, ABclonal) for 1 h at 25 C

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BDNF-Adjacent Pathways: While GHK-Cu does not directly upregulate BDNF (Brain-Derived Neurotrophic Factor), its anti-inflammatory effects in the CNS specifically its reduction of microglial activation may create conditions conducive to BDNF signaling