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Subsequently, 100 L SGID IMF or HBSS (buffer only control) was added to the apical compartment (final dilution 1 in 15) for 2 h at 37C

Dosing schedules and timing Tirzepatide is typically injected once per week, on the same day each week

doi: 10.1210/mend.14.10.0532 40 WozniakALBulayevaNNWatsonCS

Primer sequences and annealing temperatures, for Claudin-2 (CLDN-2), Junctional Adhesion Molecule 1 (JAM-1), Occludin (OCLN), Zonula Occludens-1 (ZO-1), Claudin-4 (CLDN-4), Ribosomal Protein Lateral Stalk Subunit (P0-RPLP0), Mucin-1 (MUC-1), Mucin-2 (MUC-2), Mucin-5AC (MUC-5A) cyclin-dependent kinase inhibitor 1 (p21) and tumor protein p53 (p53), are listed in Supplementary Table 1