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In terms of specific regional regulatory practices, the United States defines EV-based products as biologics, requiring submission through the Investigational New Drug (IND) application process and undergoing rigorous preclinical and quality control reviews by the US Food and Drug Administration (USFDA) [167]

Others might theoretically conflict with metabolic pathways already being modulated

Avoid repeated freeze-thaw cycles for all compounds

This property makes them particularly amenable to surface modifications, such as coating with polymers or targeting ligands, to improve drug loading capacity