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Live/Dead PI stain (Thermo Fisher Scientific) was added just before analyzing samples on a flow cytometer

In the study by Vladimir et al., tumor necrosis factor alpha (TNF-) was used to induce brain inflammation, and subsequently, liposomes modified with different ligands were injected

A significant proportion of moderate or partially deficient patients (Class 3) still developed severe hyperbilirubinemia that require prompt phototherapy intervention (14), showing that higher residual enzyme activity level does not confer more protection against hyperbilirubinemia

Taking 1 mL working solution as an example, add 100 L EtOH stock solution ( 12.5 mg/mL ) to 900 L Corn oil , and mix evenly