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The ANT-treated animals had significantly increased plasma levels of ALT, AST, and total bilirubin compared to the control group (Fig

Validation of GCMS method has been previously reported 20

Although therapies targeting this pathway have shown potential in preclinical and early clinical trials, their tissue-specific effects and long-term safety still need to be further explored

The primary endpoint for this study was the number of live offspring per round of confirmed mating, which was determined by the presence of a vaginal plug as described above, and the total number of live offspring for each cohort, with n = 10 animals per group