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greater weight loss than single-pathway GLP-1 medications

Body iron stores, calculated from blood Hb concentration, mean corpuscular volume, serum ferritin, and transferrin receptor were highly significant predictors of TSH and total T4 ( P 005) ( Reference Nekrasova, Strongin and Ledentsova 49 )

The complete nutrient medium was comprised of phenol red-containing Dulbeccos Modified Eagles medium (DMEM, Lonza) with 4.5 g/L glucose, L-glutamine and supplemented with fetal bovine serum (FBS, Sigma-Aldrich) to a 10% final concentration and penicillin/streptomycin mixture to final concentrations of 100 U/mL for each of them

Fluorescence was measured at 4, 24, 48 and 72 h after treatment at 485 nm excitation and 530 nm emission on a SpectraMax M3 microplate reader (Molecular Devices)