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RFK KO and FLAD1 KO lines were generated by transduction with lentiCRISPRv2 hygro virus encoding the appropriate sgRNA guides

They have the ability to bind to estrogen receptors and impart similar effects as the sex hormone.55 A rise in estrogen happens to correlate with an increase in the number and size of one's milk ducts, which in turn may lead to a more voluminous milk supply.56 At the same time, LactMed warns that estrogens can also decrease milk supply, especially when taken before one's milk supply is established.57 Full establishment can take up to six weeks postpartum, so you might consider delaying your use of Liquid Gold until then