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254 SURFACE functionalization MSNs have very competitive applications in various areas of biomedicine, to a large extent due to their hydrophilic surface containing a large proportion of SiOH groups, which makes them susceptible to various functionalization modifications on the external or internal porous surface

These methods optionally further involve: (3) reassembling (&/or subjecting to one or more directed evolution methods described herein) at least one optimized recombinant nucleic acid with a further form of the nucleic acid, which is the same or different from the first and second forms, to produce a further library of recombinant nucleic acids

However, 50% of yeast GRXs are oxidized at resting conditions 59 , supporting our notion that the midpoint redox potential of the active site disulfide does not determine the redox state of GRXs if they are in an environment with the interacting ligands GSH and GSSG

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