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In keeping with the general rules of physiological signaling in which regulation is an essential requirement in contrast with oxidative stress, one would expect that glutathionylation associated with signaling is often catalyzed by enzymes that use the target protein cysteine as a substrate replacing one of the GSH in the glutathione peroxidase reaction

This allowed us to observe a higher mitochondrial mass and membrane potential in aged Mda5 -/- HSCs, a characteristic of young HSCs 47,48,49 , albeit in studies utilizing verapamil (Fig