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Similarly, they reported a temporal increase in As concentration in zooplankton indicating potentially greater effect through food and their uptake (Chen and Folt 2000)

The buffy coat was removed and leucocytes isolated by Histopaque gradient density separation (Histopaque -1077, Merck, Cat No

The strains were propagated in RBC (A+) in complete medium (RPMI 1640 medium supplemented with 0.5% Albumax, 9 mM glucose, 0.2 mM hypoxanthine, 2.1 mM L-glutamine, and 22 g/ml gentamycin) at 3.3% hematocrit and 37C in a gaseous mixture consisting of 3% O 2 , 3% CO 2 , and 94% N 2