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Proteomic analysis has shown that x-ray repair cross-complementing protein 1 (XRCC1) K247 lactylation enhances its binding affinity for importin- (also known as Karyopherin-), facilitating nuclear translocation and promoting DNA damage repair in tumor cells

However under H, there were no significant differences among cell lines

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the POI is released from the ELP-intein complex, which is selectively removed by a second round of ammonium sulfate precipitation and centrifugation, giving rise to POI of high purity in solution