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In vitro Photothermal Test PTFTH suspensions were added into 96-well plates and their thermal characteristics were measured under 808 nm laser irradiation at different irradiation intensities (0.5, 1.0, 1.5, and 2.0 W/cm 2 ), with elevated concentrations (50, 100, 200, and 400 g/mL) for 10 min

Through biochemical reconstitution, AlphaFold3-guided modeling, and targeted mutagenesis, we define conserved hydrophobic and electrostatic contacts between Sororin-CTR and the RAD21-SMC3 gate, the disruption of which abolishes cohesion in a Wapl-dependent manner