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Gallstone formation is a multifactorial process influenced by bile composition imbalances, gallbladder motility disorders, and dietary factors

A good source of protein, beans and other legumes may be a good substitute for meat, which has been shown to increase inflammation

For cleavage of the N-terminal hexahistidine-tag, TEV protease was added to the pooled elution fractions and dialyzed overnight into dialysis buffer at 4 C (25 mM Tris pH 8.0, 100 mM NaCl, 4 mM MgCl 2 , 10 M GDP, 1 mM TCEP, and 5 % glycerol)

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