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doi: 10.1016/S0009-8981(00)00306-5 167 BednarekJWysockiHSowiskiJ

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Safety of every-other-day fasting in the treatment of spinal cord injury: a randomized controlled trial

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The final pellets were suspended in Laemmli sample buffer, boiled for 5 min, and then briefly centrifuged to collect the supernatants, which were then subjected to SDS-PAGE followed by immunoblotting analysis

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Toxic effects are also associated with the inhibition of key enzymatic activities, including -amylase, peptidase, malate dehydrogenase, protease, and pyruvate kinase, all of which are essential for starch hydrolysis, energy metabolism, and protein mobilization

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