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To mimic in-vitro responses observed in the organ chamber, where we stimulated Ach-dependent vasorelaxation in the presence or absence of TMAO pre-incubation, HAECs were pre-incubated for one hour with TMAO (10 6 M) followed by one hour stimulation with Ach (10 6 M)

Feb 22 2021;22(4)doi:10.3390/ijms22042188

and expanded

To date, these patients remain alive and under follow-up, with no recorded local recurrence or disease progression after a median follow-up period of 61 months