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[DOI] [PMC free article] [PubMed] [Google Scholar] 172.Khalil M., Alliger K., Weidinger C., Yerinde C., Wirtz S., Becker C., Engel M.A

Using reduced and oxidized glutathione, you can refold this protein into a more compact and more energetically favorable protein on the right where the disulfide bond is now between the first and third cysteine residues Glutathione is important in maintaining redox balance in cultured cells, and is already included in many formulations of cell media (Ishii & Mann, 2014)

Enzymatic Antioxidants Several enzymes obstruct free radicals formation, some of them act directly in scavenging ROS (primary enzymes), whereas secondary enzymes play an indirect role by supporting other endogenous antioxidants (Figure 2)