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In HpGGT, mutation of the same threonine residue at position 398 to serine (T398S) and alanine (T398A) resulted in comparable processing rates with respect to native HpGGT ( FIGURE 3 The role of residues present in the immediate vicinity of the cleavage site during autoprocessing was also explored

This metabolic shift rapidly depletes cellular glycogen reserves, generates excessive lactic acid, and leads to the crushing, unyielding fatigue that characterizes the Long COVID experience