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Limitations and Future Directions While our study establishes GPR68 as a central ferroptosis regulator, several questions remain

Although the mechanism for this upregulation is not fully characterized, altered redox states are known to enhance expression of numerous genes/proteins

It has been postulated that brewed unfiltered coffee contains much more antioxidant substances than ready-to-drink coffee used in our study as well as paper-filtered coffee, 23 possibly resulting in a larger content of antioxidant substances that were absorbed into the systemic circulation following oral administration, and hence a higher total antioxidant status in the plasma

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