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Oocyte Maturation Rate: It is generally related to nuclear maturity, being defined as the proportion of oocytes at MII stage ( Normal fertilization: Defined as the appearance of two protonuclei and two polar bodies at 16~18h after insemination ( Day three (D3) top-quality embryos: They are generally defined as those originating from normally fertilized oocytes with a cell count of 79 on day three after fertilization, the fragmentation rate is less than 10%, and there are no multinucleated embryos ( Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) ( Usable blastocyst rate: This term denotes the combined count of transferred and frozen blastocysts within a single oocyte retrieval cycle

the L1-Z1- and L2-Z2-groups may be introduced simultaneously or sequentially in any order