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Replicate RT-deletion profiles were aggregated, designating six G4 targets as true positives and five non-G4 targets as negatives (listed in Source Data), and these scores were used to generate ROC curves and AUCs under pull-down+ and pull-down- conditions to compare the sequencing depth required for berberine-G4 binding detection

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doi: 10.1056/NEJMra1600266 88