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After overnight centrifugation, the band containing the nozzles was collected using a gradient fractionator (Labconco Auto Densi-Flow), dialyzed against Tris buffer, and the nozzles were either collected by centrifugation (1 h at 366,000 g ) or further purified using 30 ml gradients of 10-40% sucrose (wt/wt)

Hence, a possible explanation for the increased sperm concentration and viability in our study might be attributed to an increased lactate production by the Sertoli cells or increased GLUT protein levels and activities, although further studies are required to validate this

Brott T, Adams HP Jr, Olinger CP, et al

Thus, the role of zinc and its regulatory mechanism in CRC requires further in-depth investigation