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Serum leptin levels were measured in a 96-well microplate using a commercial Morinaga Mouse/Rat Leptin ELISA Kit (MIoBS), with absorbance measurements at 450 nm

Goncharova, professor of internal medicine and director of the Pulmonary Vascular Disease Program

emblica genes that showed at least two of these evolutionary signatures were considered as the genes with multiple signatures of adaptive evolution (MSA)