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In response to ischemia, a sharp increase in the level of HSP70 is recorded, while its highest concentration is observed in vital parts of cells: nuclear, perinuclear space, mitochondria, and endoplasmic reticulum, which indicates the importance of chaperone 70 in protection against death (Sharp et al., 2013)

The remaining missing data were subjected to kNN imputation, as this method has been proven to provide better performance, robustness, and an overall marginal type 1 error rate [30, 31], especially when the proportion of missing data across samples for a molecular feature is relatively low (as in our datasets, with only 11.6% of imputable variables)

2020.5 min.: linear gradient from 20% to 80% B