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The transformation was made by lithium acetate and PEG method according to MatchMaker protocol (Clontech)

2c, autophagy was checked by western blot analysis, using anti-LC3 and anti-p62 antibodies and by flow cytometry, using autophagy detection kit

Characteristics and Biomarkers of Ferroptosis

Hengstler 7 , Matthias Ebert 8 , Steven Dooley 9 and Dirk Drasdo 1,3 , (1)Ifado, (2)Medical Faculty Mannheim Heidelberg University, Mannheim, (3)Inria, (4)Medical Faculty Mannheim Heidelberg University, Mannheim, Germany, (5)Ghent University, (6)University of Massachusetts Amherst, (7)Department of Toxicology, Leibniz Research Centre for Working Environment and Human Factors, Technical University Dortmund, Ardeystr 67, 44139, Dortmund, Germany, (8)Medical Faculty Mannheim, Heidelberg University, (9)University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany Background: Upon different types of liver injury, distinct patterns of fibrosis arise, such as extracellular matrix (ECM) septa connecting pericentral (CV) areas due to toxic injury