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Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4

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Broz P, Pelegrin P, Shao F

In this experiment, the OCR of acquisition normal cell system and extracellular acidification rate (ECAR) are with the establishment of base line value under CoQ10 existence and non-existent situation.Observe at its natural nutrition environment, the baseline OCR of normal cell system leads different, and is generally the function of cell physiological action in vivo