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1 Remove IGF-1 DES vial from refrigeration and allow to reach room temperature (1520 minutes) 2 Clean vial top with alcohol swab and allow to air dry completely 3 Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water) 4 Inject BAC water slowly down the side of the vial do not aim directly at the powder 5 Gently swirl in circular motions DO NOT shake vigorously as this degrades the peptide 6 Allow 23 minutes for full dissolution solution should be clear and colorless 7 Draw calculated dose into an insulin syringe for subcutaneous injection 8 Inject into abdomen, thigh, or upper arm

Leukemia 22:18641873 Yang Q, Chen L, Zhang H et al (2024) DNMT1 regulates human erythropoiesis by modulating cell cycle and endoplasmic reticulum stress in a stage-specific manner

Wan Y, Slevin E, Koyama S, Huang CK, Shetty AK, Li X

Some protocols even go up to 2,000mg for short-term intensive use