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doi: 10.1038/s41420-020-0258-3 42 OgasawaraMALiuJPelicanoHHammoudiNCroceCMKeatingMJet al

This process can lead to slower recovery from injuries, stiffness in connective tissues, and visible changes in skin quality

Figure 4 Low GH and low IGF-I Like acromegaly, GHD can be considered as a primary GH disorder, where changes on the GH/IGF-I axis are reflected by a primary association with low GH and low IGF-I ( Table 1

Aliquots of proteins from L02 cells were pre-purified with Protein A/G beads (#B23202, Bimake, TX, USA) in the presence of non-specific IgG (Santa Cruz) and then incubated with His-tag antibody and 50 L beads overnight at 4 C with continuous mixing