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The citric acid metabolites speak to mitochondrial health, with multiple markers inclusive of succinic and pyroglutamic acids pointing to glutathione status as a proxy for oxidative stress

Briefly, an aliquot of cell homogenate was incubated with the labeled substrate DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroaniline), which is cleaved by the caspase-3 enzyme and releases the chromophore pNA

It should be noted that upon transduction of the cells with the variant library, there was a ~50% loss in total variant representation (Fig

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