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TABLE 1 Causes of vitamin B12 deficiency, classified by cause

When administered, the peptide preferentially accumulates in adipose tissue, where it interacts with beta-3 adrenergic receptors on fat cell membranes and initiates metabolic signaling cascades

The reactions were carried out in 384 well PCR plates in a total volume of 4 l containing 10 M of reverse and forward primers, 2 l of SensiFAST Sybr lo ROX RT-PCR mix (Bioline, Boston, MA), and 5 ng of template cDNA (1 l)