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Description

Levels of mouse cytokines were quantified in the culture supernatants by using IL-6, IFN-, IL-12p40 specific sandwich enzyme-linked immunosorbent assay (ELISA) following the manufacturers instructions (cytokine mouse uncoated ELISA kit, Invitrogen) as described 27

GPR43 activation by SCFAs reduces intracellular ATP depletion and reactive oxygen species (ROS) production, which are critical for NLRP3 assembly (132, 133)