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(III.2.F) EXVIVO STUDY Ex vivo study on goat sublingual mucosa shows increase in permeation of drug through mucosa than that of In vitro condition because chitosan is able to break tight junction hence facilitate the passage of drug through this route .On the other hand PG will increase the pore size of the membrane along with alcohol as a result increase in Para-cellular movement of the drug .Therefore Ex- vivo data will help to predict In- vivo permeation pattern of the formulation

Study 3: Combination With Diet Independent work showed that NNMT inhibition plus a lean/low-fat diet accelerated weight and fat loss beyond diet alone, improved the lean-mass-to-body-weight ratio, and ameliorated obesity-linked liver pathologies