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Vignoli A, Tenori L, Luchinat C, Saccenti E

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Stress during pregnancy alters temporal and spatial dynamics of the maternal and offspring microbiome in a sex-specific manner

Undifferentiated hepatic cells (HepaRG) were procured from Biopredic (Saint-Gregoire, France) and were cultured based on the protocol by 2 flasks at a density of 2.6 10 4 cells/cm 2 in a culture medium composed of Williams medium E, supplemented with 10% fetal bovine serum (FBS), 2 mM Glutamax TM , 50 M hydrocortisone hemisuccinate, 5 g/mL insulin, 100 IU/mL penicillin, and 100 g/mL streptomycin at 37C and 5% CO 2