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Therefore, noting the lack of spontaneous oxidation of free GSH between the two EDTA-treated samples stored at 80 o C, this result also suggests that the differences in GSH between the heparinized plasma (Preparation Condition 1) and deproteinized EDTA-treated plasma (Preparation Condition 2) are due to spontaneous oxidation of free GSH occurring in the heparinized plasma prior to the deproteinization process

Primary cortical neurons were maintained in Neurobasal medium (Life Technologies Corporation) supplemented with 1x B-27 (Life technologies)

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