main amino acids for glutathione synthesis in Cancer Cells | Biochemistry (Moscow) I have been reading today

Determination of the Contents of GSx and GSSG The levels of cellular and extracellular GSx (amount of GSH + twice the amount of GSSG) and GSSG were assessed using a modified version of the colorimetric Tietze method adapted for 96-well microtiter plates, as previously described [38]

Oxidative stress, glutathione insufficiency, and inflammatory pathways in type 2 diabetes mellitus: implications for therapeutic interventions

The adduct was further concentrated, and excess TRaQ-G ligand was removed by desalting with Zeba spin desalting columns (7k molecular weight cutoff, 0.5 ml)

However, it's important to note that these studies typically use high-quality, bioavailable forms of glutathione