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REVIEW Open Access A multifaceted review of monosodium glutamate effects on human health and its natural remedies Li Yang 1 , Yongchao Gao 2 , Jupeng Gong 3 , Liang Peng 4 , Hesham R

Spleen cell suspensions were prepared by gently tamping the spleen through a 50-mesh stainless steel screen, and collecting the cells in balanced salt solution (BSS) supplemented with 10% heat-inactivated calf serum (Grand Island Biological Company, Montreal, Quebec, Canada)