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The cells were washed with Phosphate Buffered Saline (PBS) 2 to 3 times and subsequently fixed in 4% paraformaldehyde (Solarbio, Beijing, China) for 30 min

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Furthermore, myogenic progenitors derived from C9orf72 ALS patients displayed increased susceptibility to oxidative stress and dysregulation of mitochondrial genes, and these events were associated with mitochondrial abnormalities and toxicity (Lynch et al., 2019)