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Using metabolic markers of B12 status like methylmalonic acid and homocysteine to aid in the diagnosis of clinically manifested vitamin B12 deficiency has advantages, but also shortcomings [10,11], such as the high costs of measurements, the limited availability, and the impact of renal insufficiency on the concentrations

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Indeed, as current in vitro and in vivo toxicity testing methods are mainly used to evaluate the acute and subacute toxicity of metal-based NPs, nanotoxicity testing methods for chronic long-term NPs exposure, which are crucial for predicting chronic toxicity in humans, are still lacking

Een vroege publicatie uit 1993 beschreef de mogelijkheden, maar er bleven veel vragen openstaan: zijn de effecten herhaalbaar, in welke context bruikbaar, en hoe zit het met veiligheid en mogelijke bijwerkingen