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The lipid residue in the LC-MS injection vial was redissolved with 300 L of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20C for 2 h

Body weight change The formula for calculating the percentage change in mouse weight is: (Body weight of the next day - Body weight of the previous day) 100%/ Body weight of the previous day

Researchers subsequently learned that when the drug is broken down in the liver, it produces a potentially toxic byproduct

Notably, Lactobacillus species isolated from culturomics were matched with the metagenome relative abundance by 38.5%, still, Lactobacillus species composing MSP were not regarded as dominant in both analyses (Fig