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Then, 5 l of laccase variants (3.4 g ml 1 of LacAnc100 and 4.0 g ml 1 of LaccID) were incubated in a 1-ml final volume containing 100 mM Britton and Robinson buffer at different pH values (2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0) in a 96-deep well plate (Sarstedt, 82.1972) covered with a lid

The question do we actually understand our own process? is uncomfortable

We are also testing more efficient shimming methods, as well as better spectrum processing methods with higher fitting accuracy, in the hope of obtaining higher-quality 2 H-NMR spectra