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As seen in Supplemental Figure 4B, the sum of total intracellular hydroperoxy- and hydroxy-phospholipids following IL-13 stimulation was approximately 2 nmol/mg protein, that is, sufficient amounts to quantifiably impact the change in intracellular GSH (and the increases in GSSG) under these two conditions, as shown in Supplemental Figure 4C under similar conditions

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Importantly, unlike other currently available NADP sensors, including iNaps and Apollo-NADP + 15,16 , NAPstars respond to changes in both NADPH and NADP + and are bona fide probes for the NADP redox state (Fig