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For example, TLR1, TLR2, TLR4, TLR5, TLR6, TLR10 are indeed located on the plasma membrane, whereas TLR3, TLR7, TLR8, TLR9, and TLR11 families are intracellular TLRs expressed in the membranes of endosomes and lysosomes (Lester and Li, 2014)

Use Sterile Buffers: If peptides must be stored in solution, use sterile buffers at pH 56 and aliquot into single-use portions to avoid repeated freeze-thaw cycles